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(H) Proteins extracted from the cells treated with 100nM control or SOX2 siRNA for 48 hours were subjected to immunoblotting to examine the phosphorylation of H2AX at Ser139, which is a marker of early apoptosis. (I) SF188 cells were treated with 100nM control or SOX2 siRNA for 72 hours and immunoblotting was performed on the total protein lysates. Increased caspase 3 cleavage was observed in the siSOX2 treated cells compared to the control cells.
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